New customers must register with our service before submitting a request form
To request a service please follow the instructions below.
- Observe the Sample Submission requirements detailed under each service.
- Refer also to the 96-Well Plate Instructions below.
- Download and complete the relevant request form. Enter the required information on the form and email a copy to the GUDSF.
- Also, print and enclose a copy with your samples.
- Upon receipt of your samples, staff will email you with the unique batch number assigned to your samples. This number will form part of the results file name.
Delivery Instructions
Samples may be delivered in person directly to the facility or sent by:
Australia Post
- Protect your samples by using padded post packs.
- Enclose the batch of samples in alfoil to protect from light.
Courier
- Deliver to the GUDSF Monday to Thursday 9:30 am – 3:30 pm.
- Deliveries may be left at the University’s Science Store located on Level -1 in the same building, N34, -1.02.
Internal University Mail System
Griffith customers can use the Internal University Mail System. For prompt delivery, please attach the Courier Request form to your package.
Please address deliveries to:
Attention: Nicole Hogg
Griffith University DNA Sequencing Facility
c/o Science Store
Building N34, Science Road
Griffith University
NATHAN QLD 4111
96-Well Plate Instructions
For large batches of samples please use 96-well plates.
- Leave well H12 empty for sequencing samples and A01 empty for fragment analysis samples. This is for our internal control sample.
- Clearly mark the orientation of the plate, i.e. Top left-hand corner = well A1 and write your name on the plate.
- For full plates (95 samples) please use Applied Biosystems Micro-Amp Reaction Plates (P/N N8010560) or Axygen 96-well PCR Amplification Plate (P/N ACYPCR-96M2-HS). A discount applies to full plates – see Prices for details.
- If sending in less than 95 samples in a 96-well plate (partial plate) it is important that the samples are placed in the wells of column 1 (or Run 1) before moving on to the next consecutive column. The 3130xl automatically makes injections from every well in two consecutive columns. The 96-well plate-mapping diagram below illustrates the order of loading. Note: Blank wells may incur a charge where samples are not organized into full columns.
- Enter the sample name in the corresponding well/grid position when completing the Request Form.
Run 1 | Run 2 | Run 3 | Run 4 | Run 5 | Run 6 | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | 1 | 9 | 17 | 25 | 33 | 41 | 49 | 57 | 65 | 73 | 81 | 89 |
B | 2 | 10 | 18 | 26 | 34 | 42 | 50 | 58 | 66 | 74 | 82 | 90 |
C | 3 | 11 | 19 | 27 | 35 | 43 | 51 | 59 | 67 | 75 | 83 | 91 |
D | 4 | 12 | 20 | 28 | 36 | 44 | 52 | 60 | 68 | 76 | 84 | 92 |
E | 5 | 13 | 21 | 29 | 37 | 45 | 53 | 61 | 69 | 77 | 85 | 93 |
F | 6 | 14 | 22 | 30 | 38 | 46 | 54 | 62 | 70 | 78 | 86 | 94 |
G | 7 | 15 | 23 | 31 | 39 | 47 | 55 | 63 | 71 | 79 | 87 | 95 |
H | 8 | 16 | 24 | 32 | 40 | 48 | 56 | 64 | 72 | 80 | 88 | I.C |
Required order of sample loading into a 96-well plate.
Put samples in consecutive columns. Max 95 samples.
I.C - Internal control sample, leave well H12 empty.
Run 1 | Run 2 | Run 3 | Run 4 | Run 5 | Run 6 | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | I.C | 8 | 16 | 24 | 32 | 40 | 48 | 56 | 64 | 72 | 80 | 88 |
B | 1 | 9 | 17 | 25 | 33 | 41 | 49 | 57 | 65 | 73 | 81 | 89 |
C | 2 | 10 | 18 | 26 | 34 | 42 | 50 | 58 | 66 | 74 | 82 | 90 |
D | 3 | 11 | 19 | 27 | 35 | 43 | 51 | 59 | 67 | 75 | 83 | 91 |
E | 4 | 12 | 20 | 28 | 36 | 44 | 52 | 60 | 68 | 76 | 84 | 92 |
F | 5 | 13 | 21 | 29 | 37 | 45 | 53 | 61 | 69 | 77 | 85 | 93 |
G | 6 | 14 | 22 | 30 | 38 | 46 | 54 | 62 | 70 | 78 | 86 | 94 |
H | 7 | 15 | 23 | 31 | 39 | 47 | 55 | 63 | 71 | 79 | 87 | 95 |
Required order of sample loading into a 96-well plate.
Put samples in consecutive columns.
I.C - Internal control sample, leave well A01 empty.